Analysis of Borrelia burgdorferi IgG antibodies with a combination of IgG ELISA and VlsE C6 peptide ELISA

Measurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method

Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

Dengue Virus IgG Elisa kit

Designing an ELISA Method for Measurement of Human IgG and IgM Antibodies against SARS-CoV-2

Background and purpose: Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an indirect ELISA method was designed to measure the human IgM and IgG antibodies against SARS-CoV-2. Materials and methods: Protein sequence of nucleocapsid antigen from SARS-CoV-2 was expressed in E. coli BL21 and then was...

IgG Food Allergy Testing by ELISA/EIA

Adverse reactions to food may initiate a myriad of physiological effects in the body. These reactions may be immunologically or nonimmunologically mediated and can result in signs and symptoms ranging in severity from mild to life threatening anaphylaxis. Although the majority of severe reactions are thought to be immunological and mediated via IgG and IgA may play a role in adverse reactions t...

ELISA test to detect Chlamydophila pneumoniae IgG.

A new ELISA test (Chlamydophila pneumoniae IgG, Vircell, Spain) to detect Chlamydophila pneumoniae IgG was evaluated. The micro-immunofluorescence (MIF) test was used as reference method. Chlamydia trachomatis and Chlamydophila psittaci elementary bodies were also assayed. Two hundred and sixteen sera were included in the study: 66 from patients with peripheral arterial occlusive disease (Panel...